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ALPHABETICAL DIET

 ALPHABETICAL DIET Alphabetical diet is one in which one's take one letter diet for 26 days regularly. It starts from A and end in Z. It is basically a cooking and eating challenge and allows a variety of diet in every day life. It is not a weight loss plan. Here we discuss food and drinks available in Pakistan. LETTER 'A' APPLES Pakistan is the 10th largest apple producing country globally. The popular varities included Granny Smith, Golden Delicious, KallaKaku, Pink Lady and Red Delicious. Apples contain pectin, a natural fibre found in plants.It has a cholesterol lowering effect. Apples are low on the glycaemic index (GI) prevents diabetes. Apples are rich in polyphenols ' querctin' lower risk of hearth diseases and respiratory problems.  APRICOTS The apricot's native region is Central Asia. It is a fruit with a firm exterior and a soft interior that is smaller than a peach but looks remarkably similar. Apricots are a great food for supporting eye health sinc...
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COLUMN CHROMATOGRAPHY

 COLUMN CHROMATOGRAPHY

INTRODUCTION

Column chromatography is a technique used to separate single chemical compounds from a mixture dissolved in a fluid. It separates substances based on different absorption of compounds to the adsorbent, as molecules move through the column at different rates which allow to get separated in fractions. It is used in small and large scale top purify materials that can be used in future experiments. This method is a type of adsorption column chromatography.

PRINCIPLE 

When mobile phase and mixture is introduced that need to be separated is introduced from the top of the column. As the movement of the components of mixture is at different rates in column. The component with low adsorption and affinity travels faster as compared to high adsorption and affinity. Molecule travels faster leaves the system first where as those molecules moves slowly leaves system last.

TYPES
  1. In adsorption column chromatography technique, components mixture are adsorbed on the surface of adsorbent. This method is used for separation.
  2. In partition chromatography, both stationary and mobile phase are liquid.
  3. In ion exchange chromatography, stationary phase is always ion exchange resin.
APPLICATION
  • It is used to isolate active ingredients.
  • It is helpful in separating compounds.
  • It is used to determine drug estimation from drug formulation.
  • It is used to remove impurities.
  • It is used to isolate metabolites from biological fluid.



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